IDENTIFIKASI IKAN CUPANG (Betta imbelis) TRANSGENIK FOUNDER MEMBAWA GEN PENYANDI HORMON PERTUMBUHAN; Identification of Transgenic. Ikan transgenik F-0 dibuat dengan menggunakan metode transfeksi. Identifikasi dilakukan menggunakan metode RT-PCR. RNA total. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are.

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Pada penelitian ini, kami menggunakan metode PCR real-time kuantitatif krt-PCR untuk menganalisa sigositas pada satu strain ikan zebra Danio rerio transgenik yang membawa gen Iakn dari ikan salmon masu, Oncorhynchus masou. Dengan demikian, metode krt-PCR adalah efektif untuk penentuan sigositas secara cepat dan tepat pada ikan transgenik. Transgenic individuals were identified by polymerase chain reaction PCR and their expression levels were analyzed by reverse transcription PCR. Dengan demikian, sebuah sistem transgenik untuk memodifikasi biosistesa asam lemak pada ikan telah dikembangkan.

Data were analyzed using the comparative cycle threshold method. Tingkat ekspresi transgen yang tinggi pada jaringan ikan F2 tramsgenik diperiksa telah diperoleh.

In this experiment, we applied a quantitative real-time PCR qr-PCR method to analyze zygosity in a stable line of transgenic zebrafish Danio rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene. Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti bioteknologi perikanan.

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Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis. Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur.

Jurnal Akuakultur Indonesia Vol 2, No 1 New approaches addressing this problem could be of great help for fish biotechnologists. The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis.

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Transgemik akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia. Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi.

Recently, global food security has become a hot issue by the fransgenik in national as well as out of the country. Aquacultural output will need tranegenik be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population. This technique could be useful in the establishment of breeding programs for mass transgenic fish production and in experiments in which zygosity effect could have a functional impact.

Now, a transgenic system to study the modification of fatty acid biosynthesis pathways in fish was established. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived by mating the transgenic fish and wild-type counterpart.

This method needs relatively large amounts of fish material and is time- trannsgenik labor-intensive.

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Thus, we found that the qTR-PCR to be effective for a rapid and precise determination of zygosity in transgenic fish. This review describes various techniques of gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture.

Transgen telah ditransmisikan ke ikan generasi F2 dengan mengikuti pola segregasi Mendel. This technique has been applied to several commercially valuable species.

Persilangan antara ikan transgenik tersebut dengan ikan normal menunjukkan transmisi transgen ke keturunannya mengikuti hukum segregasi Mendel. Expression of transgene in F2 generation was varied between strains regarding as the mosaics of F0 fish.

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The transmission rate of transgene into F1 generation was 4. Jurnal Akuakultur Indonesia Publisher: Highly unsaturated fatty acids HUFAespecially eicosapentaenoic acid EPA and docosahexaenoic acid DHA have long been recognized for its beneficial effect for human health and development. Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga.

Rapid method for identification of transgenic fish zygosity Jurnal Akuakultur Indonesia Vol 6, No 2 The first F1 and second F2 generation was produced by crossing the transgenic founder F0 and F1, respectively, with wild-type fish. This Author published in this journals.

One of the advances biotechnologies that expected to be a powerful ikna for aquaculture development transtenik transgenic technique. Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Jurnal Akuakultur Indonesia Vol 6, No 1 The intensity and capacity of production is expected to increase using biotechnology approach. Data dianalisis menggunakan metode pembandingan nilai cycle threshold.

Jurnal Akuakultur Indonesia Vol 6, No 2 Teknik ini dapat berguna dalam program produksi ikan transgenik secara massal dan dalam percobaan dimana faktor sigositas memberikan pengaruh nyata.

Mating of those fish with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws.

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